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Rat EPO/Erythropoietin PicoKine® Quick ELISA Kit, Carrier-free

Selected Variant

FEK1351-carrier-free

Size: 96 Well With Removable Strips

Rat EPO/Erythropoietin PicoKine® Quick ELISA Kit (120 minutes, 96 Tests). Quantitate Rat Epo in cell culture supernatants, serum and plasma (heparin). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Erythropoietin, EPO, also known as hematopoietin or hemopoietin, is a glycoprotein hormone that controls erythropoiesis, or red blood cell production. It is a cytokine for erythrocyte (red blood cell) precursors in the bone marrow. This gene is mapped to 7q22. It is said that the EPO gene encodes a deduced 193-amino acid propolypeptide. This hormone can be found in kidney and liver. It is the hormone that regulates red blood cell production. And it plays an important role in the brain's response to neuronal injury. Whata€?s more, EPO is also involved in the wound healing process.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>108</td><td>117</td><td>110</td><td>115</td><td>112</td><td>3.64</td><td>3.2%</td></tr><tr><td>Sample 2</td><td>342</td><td>374</td><td>386</td><td>373</td><td>368</td><td>16.26</td><td>4.4%</td></tr><tr><td>Sample 3</td><td>1332</td><td>1189</td><td>1227</td><td>1391</td><td>1284</td><td>80.66</td><td>6.2%</td></tr></table>*number of samples for each test n=16.

Synonyms

Erythropoietin;Epo;

Gene Name

Erythropoietin

Gene ID

24335

UniProt

P29676

Reactivity

Rat

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: T44-K226

Tissue Specificity

Produced by kidney or liver of adult mammals and by liver of fetal or neonatal mammals.

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, serum and plasma (heparin).

Detection Range

46.9 pg/ml - 3,000 pg/ml

Sensitivity

<10 pg/ml

Function

Erythropoietin is the principal hormone involved in the regulation of erythrocyte differentiation and the maintenance of a physiological level of circulating erythrocyte mass.

Components

Description|Quantity 96-well plate|1 lyophilized Standard| Biotinylated detection antibody (50x)|100 ul Avidin-Biotin-Peroxidase Complex (30x)|400 µl Sample diluent|30 ml Antibody diluent|12 ml Avidin-Biotin-Peroxidase Diluent|12 ml Wash Buffer (25x)|20 ml Color Developing Reagent (TMB)|10 ml Stop Solution|10 ml Plate Sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

21286 MW

Specificity

Natural and recombinant rat EPO

Anticoagulant

heparin

Subcellular Location

Secreted.

Protein Name

Erythropoietin

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