Human IL-5/Interleukin-5 PicoKine® Quick ELISA Kit, Carrier-free

Human IL-5/Interleukin-5 PicoKine® Quick ELISA Kit (120 minutes, 96 Tests). Quantitate Human IL5 in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 2pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Interleukin-5 (IL-5) is also known as eosinophil differentiation factor (EDF). IL-5 is a potential candidate gene in the pathogenesis of asthma, as it is the main cytokine controlling eosinophil activity and eosinophils are pivotal in the development of airway inflammation. The predicted amino acid sequence of 134 amino acids is identical with that recently reported for human interleukin-5 but shows no significant homology with other known hemopoietic growth regulators. IL-5 is a lineage-specific hematopoietic growth factor that stimulates the production of eosinophils and eosinophil colonies from normal human bone marrow cells. IL-5 gene to human chromosome 5 at bands q23-31. Human and mouse of IL-5 share 70% amino acid sequence homology.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>9</td><td>10</td><td>10</td><td>9</td><td>9</td><td>0.5</td><td>5.5%</td></tr><tr><td>Sample 2</td><td>73</td><td>73</td><td>74</td><td>62</td><td>70</td><td>4.92</td><td>7%</td></tr><tr><td>Sample 3</td><td>238</td><td>246</td><td>219</td><td>235</td><td>234</td><td>9.81</td><td>4.1%</td></tr></table>*number of samples for each test n=16.

Synonyms

Interleukin-5;IL-5;B-cell differentiation factor I;Eosinophil differentiation factor;T-cell replacing factor;TRF;IL5;

Gene Name

Interleukin-5

Gene ID

3567

UniProt

P05113

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: I20-S134

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, serum, plasma (heparin, EDTA) and urine.

Detection Range

7.8 pg/ml - 500 pg/ml

Sensitivity

<2 pg/ml

Function

Factor that induces terminal differentiation of late- developing B-cells to immunoglobulin secreting cells.

Components

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)