Human Aggrecan/ACAN PicoKine® Quick ELISA Kit, Carrier-free

Aggrecan, also known as cartilage-specific proteoglycan core protein (CSPCP) or chondroitin sulfate proteoglycan 1, is a protein that in humans is encoded by the ACAN gene. It is a critical component for cartilage structure and the function of joints. Aggrecan provides intervertebral disc and cartilage with the ability to resist compressive loads. The localized high concentrations of aggrecan provide the osmotic properties necessary for normal tissue function with the GAGs producing the swelling pressure that counters compressive loads on the tissue. This functional ability is dependent on a high GAG/aggrecan concentration being present in the tissue extracellular matrix. Also, Aggrecan plays an important role in the organization of the extracellular spaces between neurons in the brain.

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>3084</td><td>3011</td><td>3191</td><td>2954</td><td>3060</td><td>88.56</td><td>2.8%</td></tr><tr><td>Sample 2</td><td>13064</td><td>11683</td><td>11338</td><td>13036</td><td>12280</td><td>779.41</td><td>6.3%</td></tr><tr><td>Sample 3</td><td>43102</td><td>43347</td><td>44289</td><td>42847</td><td>43396</td><td>544.9</td><td>1.2%</td></tr></table>*number of samples for each test n=16.

Aggrecan core protein;Cartilage-specific proteoglycan core protein;CSPCP;Chondroitin sulfate proteoglycan core protein 1;Chondroitin sulfate proteoglycan 1;Aggrecan core protein 2;ACAN;AGC1, CSPG1, MSK16;

This proteoglycan is a major component of extracellular matrix of cartilagenous tissues. A major function of this protein is to resist compression in cartilage. It binds avidly to hyaluronic acid via an N-terminal globular region.

Description|Quantity 96-well plate|1 lyophilized Standard| Biotinylated detection antibody (50x)|100 ul Avidin-Biotin-Peroxidase Complex (30x)|400 µl Sample diluent|30 ml Antibody diluent|12 ml Avidin-Biotin-Peroxidase Diluent|12 ml Wash Buffer (25x)|20 ml Color Developing Reagent (TMB)|10 ml Stop Solution|10 ml Plate Sealers|4

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)