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Human Angiostatin Kringle 1-3/PLG PicoKine® Quick ELISA Kit, Carrier-free

Human Angiostatin Kringle 1-3/PLG PicoKine® Quick ELISA Kit (120 minutes, 96 Tests). Quantitate Human PLG in cell culture supernatants, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Ang K1-3 is a single, non-glycosylated polypeptide chain containing 259 amino acids. It represents a proteolytic fragment of plasminogen containing the first three kringle structures. Ang K1-3 reduces endothelial cell proliferation and acts as a potent inhibitor of angiogenesis and tumor growth. It displays increased inhibitory activity (EDa?…a?€ =70 nM) relative to kringles 1-4 (EDa?…a?€ = 135 nM).

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>241</td><td>249</td><td>248</td><td>219</td><td>239</td><td>12.09</td><td>5%</td></tr><tr><td>Sample 2</td><td>1987</td><td>1892</td><td>1804</td><td>1733</td><td>1854</td><td>95.22</td><td>5.1%</td></tr><tr><td>Sample 3</td><td>5716</td><td>5780</td><td>5230</td><td>5301</td><td>5506</td><td>243.6</td><td>4.4%</td></tr></table>*number of samples for each test n=16.

Synonyms

Plasminogen;3.4.21.7;Plasmin heavy chain A;Activation peptide;Angiostatin;Plasmin heavy chain A, short form;Plasmin light chain B;PLG;

Gene Name

Plasminogen

Gene ID

5340

UniProt

P00747

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: V98-S355 (K1-3)

Tissue Specificity

Present in plasma and many other extracellular fluids. It is synthesized in the liver.

Applications

ELISA

Standard

100 ng/tube

Sample Type

cell culture supernatants, serum and plasma (heparin, EDTA)

Detection Range

156 pg/ml - 10,000 pg/ml

Sensitivity

<10 pg/ml

Function

Plasmin dissolves the fibrin of blood clots and acts as a proteolytic factor in a variety of other processes including embryonic development, tissue remodeling, tumor invasion, and inflammation. In ovulation, weakens the walls of the Graafian follicle. It activates the urokinase-type plasminogen activator, collagenases and several complement zymogens, such as C1 and C5. Cleavage of fibronectin and laminin leads to cell detachment and apoptosis. Also cleaves fibrin, thrombospondin and von Willebrand factor. Its role in tissue remodeling and tumor invasion may be modulated by CSPG4. Binds to cells. .

Components

Description|Quantity 96-well plate|1 lyophilized Standard| Biotinylated detection antibody (50x)|100 ul Avidin-Biotin-Peroxidase Complex (30x)|400 µl Sample diluent|30 ml Antibody diluent|12 ml Avidin-Biotin-Peroxidase Diluent|12 ml Wash Buffer (25x)|20 ml Color Developing Reagent (TMB)|10 ml Stop Solution|10 ml Plate Sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

90569 MW

Specificity

Natural and recombinant human Angiostatin K1-3

Anticoagulant

heparin or EDTA

Subcellular Location

Secreted . Locates to the cell surface where it is proteolytically cleaved to produce the active plasmin. Interaction with HRG tethers it to the cell surface.

Sequence Similarities

Belongs to the peptidase S1 family. Plasminogen subfamily.

Protein Name

Plasminogen

Frequently Asked Questions

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