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Human Adiponectin PicoKine® Quick ELISA Kit, Carrier-free

Selected Variant

FEK0595-carrier-free

Size: 96 Well With Removable Strips

Human Adiponectin PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human ADIPOQ in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 60pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Adiponectin (ADPN) is a hormone secreted by adipocytes that regulates energy homeostasis and glucose and lipid metabolism. Adiponectin is a new member of the family of soluble defense collagens, in hematopoiesis and immune responses. It is an important negative regulator in hematopoiesis and immune systems and raise the possibility that it may be involved in ending inflammatory responses through its inhibitory functions. Adiponectin is mapped to 3q27 and can protect the organism from systemic inflammation by promoting the clearance of early apoptotic cells by macrophages through a receptor-dependent pathway involving calreticulin. The standard product used in this kit is the product of gene recombination, consisting of 226 (19-244) amino acids with the molecular mass of 36KDa after glycosylation.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>3726</td><td>3810</td><td>3849</td><td>3865</td><td>3812</td><td>53.79</td><td>1.4%</td></tr><tr><td>Sample 2</td><td>12058</td><td>12135</td><td>11622</td><td>10357</td><td>11543</td><td>712.13</td><td>6.1%</td></tr><tr><td>Sample 3</td><td>49927</td><td>48196</td><td>52754</td><td>50855</td><td>50433</td><td>1645.09</td><td>3.2%</td></tr></table>*number of samples for each test n=16.

Synonyms

Adiponectin ;Adiponectin, C1Q and collagen domain containing ;cDNA FLJ78108, highly similar to Homo sapiens adiponectin, C1Q and collagen domain containing (ADIPOQ), mRNA ;ADIPOQ ;hCG_1784052 ;

Gene Name

adiponectin, C1Q and collagen domain containing

Gene ID

9370

UniProt

A8K660

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: E19-N244

Applications

ELISA

Standard

100 ng/tube

Sample Type

cell culture supernatants, serum, plasma (heparin, EDTA) and urine

Detection Range

1.56 ng/ml - 100 ng/ml

Sensitivity

<60 pg/ml

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x)|1|12ml Color Developing Reagent (TMB)|1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Calculated Molecular Weight

26414 MW

Specificity

Natural and recombinant Human ADIPOQ

Anticoagulant

heparin or EDTA

Protein Name

Adiponectin

Price

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