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Human IL-3 ELISA Kit EZ-Set™ (DIY Antibody Pairs), Carrier-free

Selected Variant

EZ0402-carrier-free

Size: 5 Plates/Kit

Human IL-3 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Human IL3 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

Product Specifications

Background

The gene IL-3 encodes interleukin 3, a hematopoietic colony-stimulating factor (CSF) that is capable of supporting the proliferation of a broad range of hematopoietic cell types. Interleukin-3 (IL-3), a protein of 140 amino acids, is chemically synthesized by means of an automated peptide synthesizer and is shown to have the biological activities attributed to native IL-3. The cDNA sequence for murine interleukin-3, one of the colony stimulating factors that regulate haematopoiesis, codes for a polypeptide of 166 amino acids including a putative signal peptide. The mouse IL 3 gene is located on chromosome 11. The human gene encoding IL 3 is tandemly arrayed on the long arm of chromosome 5. The standard product used in this kit is recombinant human IL-3, consisting of 133 amino acids with the molecular mass of 15KDa.

Synonyms

Interleukin-3;IL-3;Hematopoietic growth factor;Mast cell growth factor;MCGF;Multipotential colony-stimulating factor;P-cell-stimulating factor;IL3;

Gene Name

Interleukin-3

Gene ID

3562

UniProt

P08700

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: A20-F152

Tissue Specificity

Activated T-cells, mast cells, natural killer cells.

Applications

ELISA

Sample Type

cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

Detection Range

15.6 pg/ml - 1,000 pg/ml

Function

Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages.

Components

Description Capture Antibody Detection Antibody Avidin-Biotin-Peroxidase Complex (ABC) Lyophilized Standard|

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

17233 MW

Specificity

Natural and recombinant human IL-3

Anticoagulant

heparin, EDTA or citrate

Subcellular Location

Secreted.

Sequence Similarities

Belongs to the IL-3 family.

Protein Name

Interleukin-3

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