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Human IL-32 ELISA Kit EZ-Set™ (DIY Antibody Pairs), Carrier-free

Selected Variant

EZ1599-carrier-free

Size: 5 Plates/Kit

Human IL-32 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Human IL32 in cell culture supernatants, serum, plasma (heparin, EDTA).

Product Specifications

Background

Interleukin 32 (Il32) is a protein that in humans is encoded by the IL32 gene. This gene encodes a member of the cytokine family. The protein contains a tyrosine sulfation site, 3 potential N-myristoylation sites, multiple putative phosphorylation sites, and an RGD cell-attachment sequence. Expression of this protein is increased after the activation of T-cells by mitogens or the activation of NK cells by IL-2. This protein induces the production of TNFalpha from macrophage cells. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Synonyms

Interleukin-32; IL-32; Natural killer cells protein 4; Tumor necrosis factor alpha-inducing factor; IL32; NK4; TAIF

Gene Name

interleukin 32

Gene ID

9235

UniProt

P24001

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: A31-K234

Tissue Specificity

Selectively expressed in lymphocytes. Expression is more prominent in immune cells than in non-immune cells.

Applications

ELISA

Sample Type

cell culture supernatants, serum, plasma (heparin, EDTA).

Detection Range

62.5 pg/ml - 4,000 pg/ml

Function

Cytokine that may play a role in innate and adaptive immune responses. It induces various cytokines such as TNFA/TNF-alpha and IL8. It activates typical cytokine signal pathways of NF-kappa-B and p38 MAPK.

Components

Description Capture Antibody Detection Antibody Avidin-Biotin-Peroxidase Complex (ABC) Lyophilized Standard|

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human IL-32

Anticoagulant

heparin or EDTA

Subcellular Location

Secreted

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