Human Angiogenin/ANG ELISA Kit EZ-Set™ (DIY Antibody Pairs), Carrier-free

Human Angiogenin/ANG ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Human ANG in cell culture supernatants, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).

Product Specifications

Background

Angiogenin (Ang) also known as ribonuclease 5 is a protein that in humans is encoded by the ANG gene. Angiogenin is a potent stimulator of new blood vessel formation. It hydrolyzes cellular tRNAs resulting in decreased protein synthesis and is similar to pancreatic ribonuclease. Hooper et al. (2003) reviewed the evidence that angiogenins are involved in host defense and noted that inflammation provokes upregulated ANG mRNA expression in liver and an increase in detectable ANG protein in serum. Weremowicz et al. (1989, 1990) assigned the human angiogenin gene to chromosome 14q11.

Synonyms

Angiogenin;3.1.27.-;Ribonuclease 5;RNase 5;ANG;RNASE5;

Gene Name

Angiogenin

Gene ID

283

UniProt

P03950

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: Q25-P147

Tissue Specificity

Expressed predominantly in the liver. Also detected in endothelial cells and spinal cord neurons. .

Applications

ELISA

Sample Type

cell culture supernatants, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).

Detection Range

78 pg/ml - 5,000 pg/ml

Function

Binds to actin on the surface of endothelial cells; once bound, angiogenin is endocytosed and translocated to the nucleus. Stimulates ribosomal RNA synthesis including that containing the initiation site sequences of 45S rRNA. Cleaves tRNA within anticodon loops to produce tRNA-derived stress-induced fragments (tiRNAs) which inhibit protein synthesis and triggers the assembly of stress granules (SGs). Angiogenin induces vascularization of normal and malignant tissues. Angiogenic activity is regulated by interaction with RNH1 in vivo. .

Components

Description Capture Antibody Detection Antibody Avidin-Biotin-Peroxidase Complex (ABC) Lyophilized Standard|

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

16550 MW

Specificity

Natural and recombinant human Angiogenin/ANG

Anticoagulant

heparin or EDTA

Subcellular Location

Nucleus . Secreted, extracellular space, extracellular matrix, basement membrane. Nucleus, nucleolus. Rapidly endocytosed by target cells and translocated to the nucleus where it accumulates in the nucleolus and binds to DNA.