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Anti-Phospho-c-Jun (S73)+JunD (S100) Rabbit Monoclonal Antibody, Carrier-free

Boster Bio Anti-Phospho-c-Jun (S73)+JunD (S100) Rabbit Monoclonal Antibody catalog # P02038-2. Tested in WB, IHC, IP applications. This antibody reacts with Human, Mouse.

Product Specifications

Phospho Site

S73, S100

Synonyms

Transcription factor AP-1;Activator protein 1;AP1;Proto-oncogene c-Jun;V-jun avian sarcoma virus 17 oncogene homolog;p39;JUN;

Gene Name

Transcription factor AP-1

UniProt

P05412,P17535

Host

Rabbit

Reactivity

Human,Mouse

Immunogen

A synthesized peptide derived from human Phospho-c-Jun (S73)+JunD (S100)

Clonality

Monoclonal

Clone

Clone: 30J61

Tissue Specificity

Expressed in brain, and to a lesser extent in lung, kidney and testis.

Applications

WB,IHC,IP

Purification

Affinity-chromatography

Concentration

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Function

Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. Involved in activated KRAS-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells (PubMed:24623306). Binds to the USP28 promoter in colorectal cancer (CRC) cells (PubMed:24623306). .

Components

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Storage Conditions

Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

35676 MW

Observed Molecular Weight

38 kDa, 42 kDa, 45 kDa

Fragment

IgG

Applications Notes

WB 1:500-1:2000<br>IHC 1:50-1:200<br>IP 1:50

Subcellular Location

Nucleus.

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