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Anti-Phospho-PBK/TOPK (Thr9) Rabbit Monoclonal Antibody, Carrier-free

Boster Bio Anti-Phospho-PBK/TOPK (Thr9) Rabbit Monoclonal Antibody catalog # P02623. Tested in WB, IHC applications. This antibody reacts with Human.

Product Specifications

Phospho Site

Thr9

Synonyms

Adapter molecule crk;Proto-oncogene c-Crk;p38;CRK;

Gene Name

Adapter molecule crk

UniProt

Q96KB5

Host

Rabbit

Reactivity

Human

Immunogen

A synthesized peptide derived from human Phospho-PBK/TOPK (Thr9)

Clonality

Monoclonal

Clone

Clone: 30P33

Tissue Specificity

Expressed in brain, and to a lesser extent in lung, kidney and testis.

Applications

WB,IHC

Purification

Affinity-chromatography

Concentration

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Function

The Crk-I and Crk-II forms differ in their biological activities. Crk-II has less transforming activity than Crk-I. Crk- II mediates attachment-induced MAPK8 activation, membrane ruffling and cell motility in a Rac-dependent manner. Involved in phagocytosis of apoptotic cells and cell motility via its interaction with DOCK1 and DOCK4. May regulate the EFNA5-EPHA3 signaling. .

Components

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Storage Conditions

Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

33831 MW

Observed Molecular Weight

40 kDa

Fragment

IgG

Applications Notes

WB 1:500-1:2000<br>IHC 1:50-1:200

Subcellular Location

Cytoplasm . Cell membrane . Translocated to the plasma membrane upon cell adhesion. .

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