Anti-Phospho-Histone H3.3 (S31) Rabbit Monoclonal Antibody, Carrier-free

Boster Bio Anti-Phospho-Histone H3.3 (S31) Rabbit Monoclonal Antibody catalog # P06819-5. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Product Specifications

Phospho Site

S31

Synonyms

Hormone-sensitive lipase;HSL;3.1.1.79;LIPE;

Gene Name

Hormone-sensitive lipase

UniProt

P84243

Host

Rabbit

Reactivity

Human,Mouse,Rat

Immunogen

A synthesized peptide derived from human Phospho-Histone H3.3 (S31)

Clonality

Monoclonal

Clone

Clone: 28H13

Tissue Specificity

Four types of regulatory chains are found: I- alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible.

Applications

WB,IHC,ICC,IF,Flow Cytometry

Purification

Affinity-chromatography

Concentration

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Function

In adipose tissue and heart, it primarily hydrolyzes stored triglycerides to free fatty acids, while in steroidogenic tissues, it principally converts cholesteryl esters to free cholesterol for steroid hormone production.

Components

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Storage Conditions

Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

116598 MW

Observed Molecular Weight

15 kDa

Fragment

IgG

Applications Notes

WB 1:500-1:2000<br>IHC 1:50-1:200<br>ICC/IF 1:50-1:200<br>FC 1:50

Subcellular Location

Cell membrane. Membrane, caveola. Cytoplasm, cytosol. Found in the high-density caveolae. Translocates to the cytoplasm from the caveolae upon insulin stimulation.