Anti-IL-1 beta Rabbit Monoclonal Antibody, Carrier-free

Boster Bio Anti-IL-1 beta Rabbit Monoclonal Antibody catalog # M00101-3. Tested in WB, IP, Flow Cytometry applications. This antibody reacts with Mouse.

Product Specifications

Synonyms

Interleukin-1 beta;IL-1 beta;Catabolin;IL1B;IL1F2;

Gene Name

Interleukin-1 beta

UniProt

P10749

Host

Rabbit

Reactivity

Mouse

Immunogen

A synthesized peptide derived from human IL-1 beta

Clonality

Monoclonal

Clone

Clone: 28I10

Tissue Specificity

Detected in astrocytoma, neuroblastoma and adrenal cortex cell lines. Some isoforms are detected in foreskin fibroblast cell lines, however isoform 17, isoform 18 and isoform 19 are not detected in these cells. .

Applications

WB,IP,Flow Cytometry

Purification

Affinity-chromatography

Concentration

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Function

Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. .

Components

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Storage Conditions

Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

30748 MW

Observed Molecular Weight

31 kDa, 28 kDa, 17 kDa

Fragment

IgG

Applications Notes

WB 1:500-1:2000<br>IP 1:50<br>FC 1:50

Subcellular Location

Secreted. The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins.