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Anti-GRAF Rabbit Monoclonal Antibody, Carrier-free

Boster Bio Anti-GRAF Rabbit Monoclonal Antibody catalog # M08027. Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.

Product Specifications

Synonyms

Heat shock protein beta-6;HspB6;Heat shock 20 kDa-like protein p20;HSPB6;

Gene Name

Heat shock protein beta-6

UniProt

Q9UNA1

Host

Rabbit

Reactivity

Human,Mouse,Rat

Immunogen

A synthesized peptide derived from human GRAF

Clonality

Monoclonal

Clone

Clone: 27A82

Tissue Specificity

Brain.

Applications

WB,IHC

Purification

Affinity-chromatography

Concentration

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Function

E2-like enzyme involved in autophagy. Acts as an E2-like enzyme that catalyzes the conjugation of ATG12 to ATG5. ATG12 conjugation to ATG5 is required for autophagy. Likely serves as an ATG5-recognition molecule. Not involved in ATG12 conjugation to ATG3 (By similarity). Plays a role in adenovirus-mediated cell lysis. .

Components

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Storage Conditions

Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

17136 MW

Observed Molecular Weight

100 kDa

Fragment

IgG

Applications Notes

WB 1:500-1:2000<br>IHC 1:50-1:200

Subcellular Location

Cytoplasm . Nucleus . Translocates to nuclear foci during heat shock.

Protein Name

T-cell surface glycoprotein CD3 zeta chain

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