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Anti-Histone H2B (mono methyl K116) Rabbit Monoclonal Antibody, Carrier-free

Boster Bio Anti-Histone H2B (mono methyl K116) Rabbit Monoclonal Antibody catalog # M07286-3. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.

Product Specifications

Synonyms

Histone H2B type 2-E;Histone H2B-GL105;Histone H2B.q;H2B/q;HIST2H2BE;H2BFQ;

Gene Name

Histone H2B type 2-E

UniProt

Q16778

Host

Rabbit

Reactivity

Human,Mouse,Rat

Immunogen

A synthesized peptide derived from human Histone H2B (mono methyl K116)

Clonality

Monoclonal

Clone

Clone: 27H07

Tissue Specificity

Expressed on a subclass of T-lymphocytes known as intra-epithelial lymphocytes which are located between mucosal epithelial cells.

Applications

WB,IHC,ICC,IF

Purification

Affinity-chromatography

Concentration

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Function

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Components

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Storage Conditions

Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

13920 MW

Observed Molecular Weight

14 kDa

Fragment

IgG

Applications Notes

WB 1:500-1:2000<br>IHC 1:50-1:200<br>ICC/IF 1:50-1:200

Subcellular Location

Nucleus. Chromosome.

Protein Name

T-cell surface glycoprotein CD3 zeta chain

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