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Anti-Histone H3 (mutated K27 Met) Rabbit Monoclonal Antibody, Carrier-free

Boster Bio Anti-Histone H3 (mutated K27 Met) Rabbit Monoclonal Antibody catalog # M12477-14. Tested in WB, ICC/IF, IP, ChIP applications. This antibody reacts with Human.

Product Specifications

Synonyms

Histone H3.1;Histone H3/a;Histone H3/b;Histone H3/c;Histone H3/d;Histone H3/f;Histone H3/h;Histone H3/i;Histone H3/j;Histone H3/k;Histone H3/l;HIST1H3A;H3FA;HIST1H3B;H3FL;HIST1H3C;H3FC;HIST1H3D;H3FB;HIST1H3E;H3FD;HIST1H3F;H3FI;HIST1H3G;H3FH;HIST1H3H;H3FK;HIST1H3I;H3FF;HIST1H3J;H3FJ;

Gene Name

Histone H3.1

UniProt

P68431

Host

Rabbit

Reactivity

Human

Immunogen

A synthesized peptide derived from Histone H3 (mutated K27 Met)

Clonality

Monoclonal

Clone

Clone: 26H46

Tissue Specificity

Highly expressed in the brain, with higher expression during development than in adult. Expressed also in mammary glands, testis, colon and kidney. .

Applications

WB,ICC,IF,IP,ChIP

Purification

Affinity-chromatography

Concentration

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Function

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Components

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Storage Conditions

Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

15404 MW

Observed Molecular Weight

18 kDa

Fragment

IgG

Applications Notes

WB 1:500-1:2000<br>ICC/IF 1:50-1:200<br>IP 1:50<br>ChIP 1:50

Subcellular Location

Nucleus. Chromosome.

Protein Name

T-cell surface glycoprotein CD3 zeta chain

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