Anti-P4HB Rabbit Monoclonal Antibody, Carrier-free

Boster Bio Anti-P4HB Rabbit Monoclonal Antibody catalog # M02335-2. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Product Specifications

Synonyms

Catenin delta-1;Cadherin-associated Src substrate;CAS;p120 catenin;p120 (ctn);p120 (cas);CTNND1;KIAA0384;

Gene Name

Catenin delta-1

UniProt

P07237

Host

Rabbit

Reactivity

Human,Mouse,Rat

Immunogen

A synthesized peptide derived from human P4HB

Clonality

Monoclonal

Clone

Clone: 25P61

Tissue Specificity

Expressed in vascular endothelium. Melanocytes and melanoma cells primarily express the long isoform 1A, whereas keratinocytes express shorter isoforms, especially 3A. The shortest isoform 4A, is detected in normal keratinocytes and melanocytes, and generally lost from cells derived from squamous cell carcinomas or melanomas. The C-terminal alternatively spliced exon B is present in the p120ctn transcripts in the colon, intestine and prostate, but lost in several tumor tissues derived from these organs. .

Applications

WB,IHC,ICC,IF,Flow Cytometry

Purification

Affinity-chromatography

Concentration

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Function

Binds to and inhibits the transcriptional repressor ZBTB33, which may lead to activation of target genes of the Wnt signaling pathway (By similarity). Associates with and regulates the cell adhesion properties of both C-, E- and N-cadherins, being critical for their surface stability. Implicated both in cell transformation by SRC and in ligand-induced receptor signaling through the EGF, PDGF, CSF-1 and ERBB2 receptors. Promotes GLIS2 C-terminal cleavage. .

Components

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Storage Conditions

Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

108170 MW

Observed Molecular Weight

57 kDa

Fragment

IgG

Applications Notes

WB 1:500-1:2000<br>IHC 1:100-1:500<br>ICC/IF 1:50-1:200<br>FC 1:50

Subcellular Location

Cytoplasm. Nucleus. Cell membrane. Interaction with GLIS2 promotes nuclear translocation (By similarity). Detected at cell-cell contacts. NANOS1 induces its translocation from sites of cell-cell contact to the cytoplasm. Isoforms 4A and 1AB are excluded from the nucleus. .