Anti-DCTN3 Rabbit Monoclonal Antibody, Carrier-free

Boster Bio Anti-DCTN3 Rabbit Monoclonal Antibody catalog # M11728. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.

Product Specifications

Synonyms

E3 ubiquitin-protein ligase ZNRF2;6.3.2.-;Protein Ells2;RING finger protein 202;Zinc/RING finger protein 2;ZNRF2;RNF202;

Gene Name

E3 ubiquitin-protein ligase ZNRF2

UniProt

O75935

Host

Rabbit

Reactivity

Human,Mouse,Rat

Immunogen

A synthesized peptide derived from human DCTN3

Clonality

Monoclonal

Clone

Clone: 24D73

Tissue Specificity

Highly expressed in the brain, with higher expression during development than in adult. Expressed also in mammary glands, testis, colon and kidney. .

Applications

WB,IHC,ICC,IF

Purification

Affinity-chromatography

Concentration

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Function

May play a role in the establishment and maintenance of neuronal transmission and plasticity via its ubiquitin ligase activity. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then ly transfer the ubiquitin to targeted substrates. .

Components

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Storage Conditions

Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

24115 MW

Observed Molecular Weight

21 kDa

Fragment

IgG

Applications Notes

WB 1:500-1:2000<br>IHC 1:50-1:200<br>ICC/IF 1:50-1:200

Subcellular Location

Endosome membrane ; Peripheral membrane protein . Lysosome membrane ; Peripheral membrane protein . Cell junction, synapse, presynaptic cell membrane ; Peripheral membrane protein . Present in presynaptic plasma membranes in neurons.