Anti-VPS11 Rabbit Monoclonal Antibody, Carrier-free

Boster Bio Anti-VPS11 Rabbit Monoclonal Antibody catalog # M05575. Tested in WB, IHC, Flow Cytometry applications. This antibody reacts with Human, Mouse.

Product Specifications

Synonyms

Drebrin;Developmentally-regulated brain protein;DBN1;D0S117E;

Gene Name

Drebrin

UniProt

Q9H270

Host

Rabbit

Reactivity

Human,Mouse

Immunogen

A synthesized peptide derived from human VPS11

Clonality

Monoclonal

Clone

Clone: 22V84

Tissue Specificity

Brain neurons. Also found in the heart, placenta, skeletal muscle, kidney and pancreas. Expressed in peripheral blood lymphocytes, including T-cells (at protein level). .

Applications

WB,IHC,Flow Cytometry

Purification

Affinity-chromatography

Concentration

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Function

Drebrins might play some role in cell migration, extension of neuronal processes and plasticity of dendrites. Required for actin polymerization at immunological synapses (IS) and for CXCR4 recruitment to IS. .

Components

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Storage Conditions

Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

71429 MW

Observed Molecular Weight

108 kDa

Fragment

IgG

Applications Notes

WB 1:500-1:2000<br>IHC 1:50-1:200<br>FC 1:80

Subcellular Location

Cytoplasm . Cytoplasm, cell cortex . Cell junction . Cell projection . Cell projection, growth cone . In the absence of antigen, evenly distributed throughout subcortical regions of the T-cell membrane and cytoplasm. In the presence of antigen, distributes to the immunological synapse forming at the T-cell-APC contact area, where it localizes at the peripheral and distal supramolecular activation clusters (SMAC) (PubMed:20215400). Colocalized with DBN1, RUFY3 and F-actin at the transitional domain of the axonal growth cone (By similarity). .