Anti-PHAPI2 / APRIL Rabbit Monoclonal Antibody, Carrier-free

Boster Bio Anti-PHAPI2 / APRIL Rabbit Monoclonal Antibody catalog # M05822. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Product Specifications

Synonyms

Cell division cycle protein 23 homolog;Anaphase-promoting complex subunit 8;APC8;Cyclosome subunit 8;CDC23;ANAPC8;

Gene Name

Cell division cycle protein 23 homolog

UniProt

Q92688

Host

Rabbit

Reactivity

Human,Mouse,Rat

Immunogen

A synthesized peptide derived from human PHAPI2 / APRIL

Clonality

Monoclonal

Clone

Clone: 22A78

Tissue Specificity

Expressed in all tissues examined.

Applications

WB,IHC,ICC,IF,Flow Cytometry

Purification

Affinity-chromatography

Concentration

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Function

Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. .

Components

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Storage Conditions

Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

68834 MW

Observed Molecular Weight

29 kDa

Fragment

IgG

Applications Notes

WB 1:500-1:1000<br>IHC 1:50-1:200<br>ICC/IF 1:50-1:200<br>FC 1:100

Subcellular Location

Cell membrane ; Lipid-anchor ; Cytoplasmic side . Endoplasmic reticulum membrane . Golgi apparatus membrane . Late endosome. Cytoplasmic vesicle, phagosome membrane ; Lipid-anchor ; Cytoplasmic side . Cytoplasmic vesicle, phagosome. Colocalizes with OSBPL1A at the late endosome. Recruited to phagosomes containing S.aureus or M.tuberculosis.