Anti-CD97 Rabbit Monoclonal Antibody, Carrier-free

Boster Bio Anti-CD97 Rabbit Monoclonal Antibody catalog # M02982-1. Tested in WB, IHC, Flow Cytometry applications. This antibody reacts with Human, Rat.

Product Specifications

Synonyms

Golgi membrane protein 1;Golgi membrane protein GP73;Golgi phosphoprotein 2;GOLM1;C9orf155, GOLPH2;PSEC0242, UNQ686/PRO1326;

Gene Name

Golgi membrane protein 1

UniProt

P48960

Host

Rabbit

Reactivity

Human,Rat

Immunogen

A synthesized peptide derived from human CD97

Clonality

Monoclonal

Clone

Clone: 22C39

Tissue Specificity

Widely expressed. Highly expressed in colon, prostate, trachea and stomach. Expressed at lower level in testis, muscle, lymphoid tissues, white blood cells and spleen. Predominantly expressed by cells of the epithelial lineage. Expressed at low level in normal liver. Expression significantly increases in virus (HBV, HCV) infected liver. Expression does not increase in liver disease due to non-viral causes (alcohol-induced liver disease, autoimmune hepatitis). Increased expression in hepatocytes appears to be a general feature of advanced liver disease. In liver tissue from patients with adult giant-cell hepatitis (GCH), it is strongly expressed in hepatocytes-derived syncytial giant cells. Constitutively expressed by biliary epithelial cells but not by hepatocytes. .

Applications

WB,IHC,Flow Cytometry

Purification

Affinity-chromatography

Concentration

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Function

Unknown. Cellular response protein to viral infection.

Components

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Storage Conditions

Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

45333 MW

Observed Molecular Weight

55 kDa, 28 kDa

Fragment

IgG

Applications Notes

WB 1:500-1:2000<br>IHC 1:50-1:200<br>FC 1:50

Subcellular Location

Golgi apparatus, cis-Golgi network membrane ; Single-pass type II membrane protein . Early Golgi. Cycles via the cell surface and endosomes upon lumenal pH disruption.