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Anti-U2AF65 Rabbit Monoclonal Antibody, Carrier-free

Boster Bio Anti-U2AF65 Rabbit Monoclonal Antibody catalog # M03639-2. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.

Product Specifications

Synonyms

Cytochrome c oxidase subunit 2;Cytochrome c oxidase polypeptide II;MT-CO2;COII, COXII, MTCO2;

Gene Name

Cytochrome c oxidase subunit 2

UniProt

P26368

Host

Rabbit

Reactivity

Human,Mouse,Rat

Immunogen

A synthesized peptide derived from human U2AF65

Clonality

Monoclonal

Clone

Clone: 21U21

Tissue Specificity

Widely expressed with highest levels of expression in liver and heart. Expressed at higher levels in cancer cell lines (e.g. A-549 and HeLa) than in normal cell lines (e.g. HEK293). .

Applications

WB,IHC,ICC,IF

Purification

Affinity-chromatography

Concentration

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Function

Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Subunits 1- 3 form the functional core of the enzyme complex. Subunit 2 transfers the electrons from cytochrome c via its binuclear copper A center to the bimetallic center of the catalytic subunit 1.

Components

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Storage Conditions

Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

25565 MW

Observed Molecular Weight

65 kDa

Fragment

IgG

Applications Notes

WB 1:500-1:2000<br>IHC 1:50-1:200<br>ICC/IF 1:50-1:200

Subcellular Location

Mitochondrion inner membrane; Multi-pass membrane protein.

Protein Name

T-cell surface glycoprotein CD3 zeta chain

Frequently Asked Questions

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