Anti-Dkk3 Rabbit Monoclonal Antibody, Carrier-free

Boster Bio Anti-Dkk3 Rabbit Monoclonal Antibody catalog # M02248-1. Tested in WB, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.

Product Specifications

Synonyms

Oligodendrocyte transcription factor 2;Oligo2;Class B basic helix-loop-helix protein 1;bHLHb1;Class E basic helix-loop-helix protein 19;bHLHe19;Protein kinase C-binding protein 2;Protein kinase C-binding protein RACK17;OLIG2;BHLHB1, BHLHE19, PRKCBP2, RACK17;

Gene Name

Oligodendrocyte transcription factor 2

UniProt

Q9UBP4

Host

Rabbit

Reactivity

Human,Mouse,Rat

Immunogen

A synthesized peptide derived from human Dkk3

Clonality

Monoclonal

Clone

Clone: 21D17

Tissue Specificity

Expressed in the brain, in oligodendrocytes. Strongly expressed in oligodendrogliomas, while expression is weak to moderate in astrocytomas. Expression in glioblastomas highly variable. .

Applications

WB,ICC,IF,IP

Purification

Affinity-chromatography

Concentration

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Function

Required for oligodendrocyte and motor neuron specification in the spinal cord, as well as for the development of somatic motor neurons in the hindbrain. Cooperates with OLIG1 to establish the pMN domain of the embryonic neural tube. Antagonist of V2 interneuron and of NKX2-2-induced V3 interneuron development (By similarity). .

Components

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Storage Conditions

Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

32385 MW

Observed Molecular Weight

60 kDa

Fragment

IgG

Applications Notes

WB 1:500-1:2000<br>ICC/IF 1:50-1:200<br>IP 1:50

Subcellular Location

Nucleus . Cytoplasm . The NLS contained in the bHLH domain could be masked in the native form and translocation to the nucleus could be mediated by interaction either with class E bHLH partner protein or with NKX2-2. .