Anti-RNF40 Rabbit Monoclonal Antibody, Carrier-free

Boster Bio Anti-RNF40 Rabbit Monoclonal Antibody catalog # M06979-1. Tested in WB, IHC, IP applications. This antibody reacts with Human, Mouse, Rat.

Product Specifications

Synonyms

MAD2L1-binding protein;Caught by MAD2 protein;MAD2L1BP;CMT2, KIAA0110;

Gene Name

MAD2L1-binding protein

UniProt

O75150

Host

Rabbit

Reactivity

Human,Mouse,Rat

Immunogen

A synthesized peptide derived from human RNF40

Clonality

Monoclonal

Clone

Clone: 21R04

Tissue Specificity

Expressed in a discontinuous manner in the basal cell layer of adult skin epidermis, but continuously in the basal layer of fetal skin epidermis and nail. Also expressed in the outer root sheath above the hair bulb in hair follicle (at protein level). Expressed homogeneously in all cell layers of the esophagus and exocervix, but detected in the basal cell layer only of oral mucosa, skin and in the basal plus the next two layers of the suprabasal epithelium of the palate. .

Applications

WB,IHC,IP

Purification

Affinity-chromatography

Concentration

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Function

May function to silence the spindle checkpoint and allow mitosis to proceed through anaphase by binding MAD2L1 after it has become dissociated from the MAD2L1-CDC20 complex. .

Components

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Storage Conditions

Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

31052 MW

Observed Molecular Weight

130 kDa

Fragment

IgG

Applications Notes

WB 1:500-1:2000<br>IHC 1:50-1:200<br>IP 1:50

Subcellular Location

Nucleus. Cytoplasm, cytoskeleton, spindle. During early mitosis, unevenly distributed throughout the nucleoplasm. From metaphase to anaphase, concentrated on the spindle.