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Anti-CD27 Rabbit Monoclonal Antibody, Carrier-free

Selected Variant

M01148-3-carrier-free

Size: 100 µL

Boster Bio Anti-CD27 Rabbit Monoclonal Antibody catalog # M01148-3. Tested in WB, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Rat.

Product Specifications

Synonyms

NF-kappa-B inhibitor alpha;I-kappa-B-alpha;IkB-alpha;IkappaBalpha;Major histocompatibility complex enhancer-binding protein MAD3;NFKBIA;IKBA, MAD3, NFKBI;

Gene Name

NF-kappa-B inhibitor alpha

UniProt

P26842

Host

Rabbit

Reactivity

Human,Rat

Immunogen

A synthesized peptide derived from human CD27

Clonality

Monoclonal

Clone

Clone: 20C54

Tissue Specificity

Expressed in brain and skeletal muscle. .

Applications

WB,ICC,IF,Flow Cytometry

Purification

Affinity-chromatography

Concentration

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Function

Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription. .

Components

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Storage Conditions

Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

35609 MW

Observed Molecular Weight

102 kDa

Fragment

IgG

Applications Notes

WB 1:500-1:2000<br>ICC/IF 1:50-1:200<br>FC 1:50

Subcellular Location

Cytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm by a nuclear localization signal (NLS) and a CRM1-dependent nuclear export. .

Protein Name

Glycogen synthase kinase-3 beta (gsk3b)

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