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Anti-Eph receptor B1 Rabbit Monoclonal Antibody, Carrier-free

Selected Variant

M02811-1-carrier-free

Size: 100 µL

Boster Bio Anti-Eph receptor B1 Rabbit Monoclonal Antibody catalog # M02811-1. Tested in WB, IP applications. This antibody reacts with Human, Mouse, Rat.

Product Specifications

Synonyms

Ubiquitin-like modifier-activating enzyme 1;6.2.1.45 ;Protein A1S9;Ubiquitin-activating enzyme E1;UBA1;A1S9T, UBE1;

Gene Name

Ubiquitin-like modifier-activating enzyme 1

UniProt

P54762

Host

Rabbit

Reactivity

Human,Mouse,Rat

Immunogen

A synthesized peptide derived from human Eph receptor B1

Clonality

Monoclonal

Clone

Clone: 20E05

Tissue Specificity

Detected in erythrocytes (at protein level). Ubiquitous. .

Applications

WB,IP

Purification

Affinity-chromatography

Concentration

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Function

Catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation through the ubiquitin- proteasome system (PubMed:1606621, PubMed:1447181). Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ubiquitin-E1 thioester and free AMP (PubMed:1447181). Essential for the formation of radiation- induced foci, timely DNA repair and for response to replication stress. Promotes the recruitment of TP53BP1 and BRCA1 at DNA damage sites (PubMed:22456334). .

Components

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Storage Conditions

Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

117849 MW

Observed Molecular Weight

120 kDa

Fragment

IgG

Applications Notes

WB 1:500-1:2000<br>IP 1:50

Subcellular Location

Cytoplasm . Mitochondrion . Nucleus .

Protein Name

T-cell surface glycoprotein CD3 zeta chain

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