Anti-ADAMTS13 Rabbit Monoclonal Antibody, Carrier-free

Boster Bio Anti-ADAMTS13 Rabbit Monoclonal Antibody catalog # M00586-1. Tested in WB, Flow Cytometry applications. This antibody reacts with Human.

Product Specifications

Synonyms

Serine/threonine-protein kinase ULK1;2.7.11.1;Autophagy-related protein 1 homolog;ATG1;hATG1;Unc-51-like kinase 1;ULK1;KIAA0722;

Gene Name

Serine/threonine-protein kinase ULK1

UniProt

Q76LX8

Host

Rabbit

Reactivity

Human

Immunogen

A synthesized peptide derived from human ADAMTS13

Clonality

Monoclonal

Clone

Clone: 20A01

Tissue Specificity

Ubiquitously expressed. Detected in the following adult tissues: skeletal muscle, heart, pancreas, brain, placenta, liver, kidney, and lung.

Applications

WB,Flow Cytometry

Purification

Affinity-chromatography

Concentration

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Function

Serine/threonine-protein kinase involved in autophagy in response to starvation. Acts upstream of phosphatidylinositol 3- kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes. Part of regulatory feedback loops in autophagy: acts both as a downstream effector and negative regulator of mammalian target of rapamycin complex 1 (mTORC1) via interaction with RPTOR. Activated via phosphorylation by AMPK and also acts as a regulator of AMPK by mediating phosphorylation of AMPK subunits PRKAA1, PRKAB2 and PRKAG1, leading to negatively regulate AMPK activity. May phosphorylate ATG13/KIAA0652 and RPTOR; however such data need additional evidences. Plays a role early in neuronal differentiation and is required for granule cell axon formation. May also phosphorylate SESN2 and SQSTM1 to regulate autophagy (PubMed:25040165). .

Components

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Storage Conditions

Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

112631 MW

Observed Molecular Weight

154 kDa

Fragment

IgG

Applications Notes

WB 1:500-1:2000<br>FC 1:100

Subcellular Location

Cytoplasm, cytosol . Preautophagosomal structure . Under starvation conditions, is localized to puncate structures primarily representing the isolation membrane that sequesters a portion of the cytoplasm resulting in the formation of an autophagosome. .