Anti-Ube2N / Ubc13 Rabbit Monoclonal Antibody, Carrier-free

Boster Bio Anti-Ube2N / Ubc13 Rabbit Monoclonal Antibody catalog # M02462-1. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.

Product Specifications

Synonyms

Major prion protein;PrP;ASCR;PrP27-30;PrP33-35C;CD230;PRNP;ALTPRP, PRIP, PRP;

Gene Name

Major prion protein

UniProt

P61088

Host

Rabbit

Reactivity

Human,Mouse,Rat

Immunogen

A synthesized peptide derived from human Ube2N / Ubc13

Clonality

Monoclonal

Clone

Clone: 19U87

Tissue Specificity

Ubiquitous.

Applications

WB,IHC,ICC,IF

Purification

Affinity-chromatography

Concentration

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Function

May play a role in neuronal development and synaptic plasticity. May be required for neuronal myelin sheath maintenance. May play a role in iron uptake and iron homeostasis. Soluble oligomers are toxic to cultured neuroblastoma cells and induce apoptosis (in vitro). Association with GPC1 (via its heparan sulfate chains) targets PRNP to lipid rafts. Also provides Cu (2+) or ZN (2+) for the ascorbate-mediated GPC1 deaminase degradation of its heparan sulfate side chains (By similarity). .

Components

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Storage Conditions

Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

27661 MW

Observed Molecular Weight

17 kDa

Fragment

IgG

Applications Notes

WB 1:500-1:2000<br>IHC 1:50-1:200<br>ICC/IF 1:50-1:200

Subcellular Location

Cell membrane; Lipid-anchor, GPI-anchor. Golgi apparatus. Targeted to lipid rafts via association with the heparan sulfate chains of GPC1. Colocates, in the presence of CU (2+), to vesicles in para- and perinuclear regions, where both proteins undergo internalization. Heparin displaces PRNP from lipid rafts and promotes endocytosis.