Anti-HSD11B1 Rabbit Monoclonal Antibody, Carrier-free

Boster Bio Anti-HSD11B1 Rabbit Monoclonal Antibody catalog # M01565-1. Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.

Product Specifications

Synonyms

Mitotic checkpoint serine/threonine-protein kinase BUB1 beta;2.7.11.1;MAD3/BUB1-related protein kinase;hBUBR1;Mitotic checkpoint kinase MAD3L;Protein SSK1;BUB1B;BUBR1, MAD3L, SSK1;

Gene Name

Mitotic checkpoint serine/threonine-protein kinase BUB1 beta

UniProt

P28845

Host

Rabbit

Reactivity

Human,Mouse,Rat

Immunogen

A synthesized peptide derived from human HSD11B1

Clonality

Monoclonal

Clone

Clone: 17H86

Tissue Specificity

Highly expressed in thymus followed by spleen. Preferentially expressed in tissues with a high mitotic index. .

Applications

WB,IHC

Purification

Affinity-chromatography

Concentration

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Function

Essential component of the mitotic checkpoint. Required for normal mitosis progression. The mitotic checkpoint delays anaphase until all chromosomes are properly attached to the mitotic spindle. One of its checkpoint functions is to inhibit the activity of the anaphase-promoting complex/cyclosome (APC/C) by blocking the binding of CDC20 to APC/C, independently of its kinase activity. The other is to monitor kinetochore activities that depend on the kinetochore motor CENPE. Required for kinetochore localization of CENPE. Negatively regulates PLK1 activity in interphase cells and suppresses centrosome amplification. Also implicated in triggering apoptosis in polyploid cells that exit aberrantly from mitotic arrest. May play a role for tumor suppression. .

Components

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Storage Conditions

Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

119545 MW

Observed Molecular Weight

69 kDa

Fragment

IgG

Applications Notes

WB 1:500-1:2000<br>IHC 1:50-1:200

Subcellular Location

Cytoplasm. Nucleus. Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasmic in interphase cells. Associates with the kinetochores in early prophase. Kinetochore localization requires BUB1, PLK1 and CASC5.