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Anti-Eg5 Rabbit Monoclonal Antibody, Carrier-free

Selected Variant

M01754-3-carrier-free

Size: 100 µL

Boster Bio Anti-Eg5 Rabbit Monoclonal Antibody catalog # M01754-3. Tested in WB, ICC/IF, IP applications. This antibody reacts with Human.

Product Specifications

Synonyms

Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial;1.3.5.1;Flavoprotein subunit of complex II;Fp;SDHA;SDH2, SDHF;

Gene Name

Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial

UniProt

P52732

Host

Rabbit

Reactivity

Human

Immunogen

A synthesized peptide derived from human Eg5

Clonality

Monoclonal

Clone

Clone: 17K83

Tissue Specificity

Expressed in cerebral cortex, basal ganglia, hippocampus, hypophysis, and optic nerve. Weakly expressed in brain stem and diencephalon. Stronger expression was detected in gray matter of frontal lobe compared to white matter (at protein level). Component of the microvilli of intestinal epithelial cells. Preferentially expressed in astrocytes of hippocampus, frontal cortex, thalamus, parahippocampal cortex, amygdala, insula, and corpus callosum. Not detected in neurons in most tissues studied. .

Applications

WB,ICC,IF,IP

Purification

Affinity-chromatography

Concentration

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Function

Flavoprotein (FP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q). Can act as a tumor suppressor. .

Components

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Storage Conditions

Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

72692 MW

Observed Molecular Weight

73 kDa

Fragment

IgG

Applications Notes

WB 1:500-1:2000<br>ICC/IF 1:50-1:200<br>IP 1:50

Subcellular Location

Mitochondrion inner membrane; Peripheral membrane protein; Matrix side.

Protein Name

Lysosome-associated membrane glycoprotein 2 (Lamp2)

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