Anti-Phospho-Tau (S324) Rabbit Monoclonal Antibody, Carrier-free

Boster Bio Anti-Phospho-Tau (S324) Rabbit Monoclonal Antibody catalog # P00097-3. Tested in WB, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.

Product Specifications

Phospho Site

S324

Synonyms

Microtubule-associated protein tau;Neurofibrillary tangle protein;Paired helical filament-tau;PHF-tau;MAPT;MAPTL, MTBT1, TAU;

Gene Name

Microtubule-associated protein tau

UniProt

P10636

Host

Rabbit

Reactivity

Human,Mouse,Rat

Immunogen

A synthesized peptide derived from human Tau

Clonality

Monoclonal

Clone

Clone: 17M40

Tissue Specificity

Expressed in neurons. Isoform PNS-tau is expressed in the peripheral nervous system while the others are expressed in the central nervous system.

Applications

WB,ICC,IF,IP

Purification

Affinity-chromatography

Concentration

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Function

Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N- terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization. .

Components

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Storage Conditions

Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

78928 MW

Observed Molecular Weight

50-70 kDa

Fragment

IgG

Applications Notes

WB 1:1000-1:5000<br>ICC/IF 1:50-1:200<br>IP 1:50

Subcellular Location

Cytoplasm, cytosol . Cell membrane ; Peripheral membrane protein ; Cytoplasmic side . Cytoplasm, cytoskeleton . Cell projection, axon . Mostly found in the axons of neurons, in the cytosol and in association with plasma membrane components.