Anti-Apg12(Atg12) Rabbit Monoclonal Antibody, Carrier-free

Boster Bio Anti-Apg12(Atg12) Rabbit Monoclonal Antibody catalog # M00820-2. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human.

Product Specifications

Synonyms

Ubiquitin-like protein ATG12;Autophagy-related protein 12;APG12-like;ATG12;APG12, APG12L;

Gene Name

Ubiquitin-like protein ATG12

UniProt

O94817

Host

Rabbit

Reactivity

Human

Immunogen

A synthesized peptide derived from human Apg12(Atg12)

Clonality

Monoclonal

Clone

Clone: 17A34

Tissue Specificity

Ubiquitous. .

Applications

WB,IHC,ICC,IF,IP,Flow Cytometry

Purification

Affinity-chromatography

Concentration

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Function

Ubiquitin-like protein involved in autophagy vesicles formation. Conjugation with ATG5 through a ubiquitin-like conjugating system involving also ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme, is essential for its function. The ATG12-ATG5 conjugate acts as an E3-like enzyme which is required for lipidation of ATG8 family proteins and their association to the vesicle membranes. The ATG12-ATG5 conjugate also regulates negatively the innate antiviral immune response by blocking the type I IFN production pathway through association with RARRES3 and MAVS. Plays also a role in translation or delivery of incoming viral RNA to the translation apparatus. .

Components

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Storage Conditions

Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

15113 MW

Observed Molecular Weight

95 kDa

Fragment

IgG

Applications Notes

WB 1:500-1:2000<br>IHC 1:50-1:200<br>ICC/IF 1:50-1:200<br>IP 1:50<br>FC 1:100

Subcellular Location

Cytoplasm . Preautophagosomal structure membrane ; Peripheral membrane protein . TECPR1 recruits the ATG12-ATG5 conjugate to the autolysosomal membrane.