Anti-GFP-Tag Mouse Monoclonal Antibody, Carrier-free

Boster Bio Anti-GFP-Tag Mouse Monoclonal Antibody catalog # M30972-1. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.

Product Specifications

Synonyms

Red fluorescent protein eqFP611;GFP-like chromoprotein;

Gene Name

Red fluorescent protein eqFP611

UniProt

P42212

Host

Mouse

Reactivity

Human,Mouse,Rat

Immunogen

Full-length GFP protein

Clonality

Monoclonal

Clone

Clone: 17G06

Tissue Specificity

Highly and selectively expressed by undifferentiated rather than differentiated embryonic stem cells (ESC). Levels rapidly diminish as soon as ESC's differentiate (at protein levels). Expressed in almost all epithelial cell membranes but not on mesodermal or neural cell membranes. Found on the surface of adenocarcinoma. .

Applications

WB,IHC,ICC,IF,IP

Purification

Affinity-chromatography

Concentration

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Function

Pigment protein. .

Components

Mouse IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Storage Conditions

Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

26053 MW

Observed Molecular Weight

50 kDa

Fragment

IgG1

Applications Notes

WB 1:3000-1:10000<br>IHC 1:50-1:200<br>ICC/IF 1:50-1:200<br>IP 1:100

Subcellular Location

Lateral cell membrane ; Single-pass type I membrane protein . Cell junction, tight junction . Colocalizes with CLDN7 at the lateral cell membrane and tight junction. .