Anti-Desmoglein 2 Monoclonal Antibody, Carrier-free

Boster Bio Anti-Desmoglein 2 Monoclonal Antibody catalog # M02035. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Product Specifications

Synonyms

Melanoma antigen recognized by T-cells 1;MART-1;Antigen LB39-AA;Antigen SK29-AA;Protein Melan-A;MLANA;MART1;

Gene Name

Melanoma antigen recognized by T-cells 1

UniProt

Q14126

Host

Rabbit

Reactivity

Human,Mouse,Rat

Immunogen

A synthesized peptide derived from human Desmoglein 2 Component of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion.

Clonality

Monoclonal

Clone

Clone: AFBF-4

Tissue Specificity

Expression is restricted to melanoma and melanocyte cell lines and retina.

Applications

WB,IHC,ICC,IF,Flow Cytometry

Purification

Affinity-chromatography

Concentration

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Function

Involved in melanosome biogenesis by ensuring the stability of GPR143. Plays a vital role in the expression, stability, trafficking, and processing of melanocyte protein PMEL, which is critical to the formation of stage II melanosomes. .

Components

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Storage Conditions

Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

13157 MW

Observed Molecular Weight

42 kDa

Fragment

Rabbit IgG

Applications Notes

WB 1:1000-1:5000<br>IHC 1:50-1:200<br>ICC/IF 1:50-1:200<br>FC 1:100

Subcellular Location

Endoplasmic reticulum membrane; Single-pass type III membrane protein. Golgi apparatus. Golgi apparatus, trans-Golgi network membrane. Melanosome. Also found in small vesicles and tubules dispersed over the entire cytoplasm. A small fraction of the protein is inserted into the membrane in an inverted orientation. Inversion of membrane topology results in the relocalization of the protein from a predominant Golgi/post- Golgi area to the endoplasmic reticulum. Melanoma cells expressing the protein with an inverted membrane topology are more effectively recognized by specific cytolytic T-lymphocytes than those expressing the protein in its native membrane orientation.