Anti-PMP70 ABCD3 Monoclonal Antibody, Carrier-free

Boster Bio Anti-PMP70 ABCD3 Monoclonal Antibody catalog # M02370. Tested in WB, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Product Specifications

Synonyms

Phosphatidylinositol 3-kinase catalytic subunit type 3;PI3-kinase type 3;PI3K type 3;PtdIns-3-kinase type 3;2.7.1.137;Phosphatidylinositol 3-kinase p100 subunit;Phosphoinositide-3-kinase class 3;hVps34;PIK3C3;VPS34;

Gene Name

Phosphatidylinositol 3-kinase catalytic subunit type 3

UniProt

P28288

Host

Rabbit

Reactivity

Human,Mouse,Rat

Immunogen

A synthesized peptide derived from human PMP70

Clonality

Monoclonal

Clone

Clone: AFAF-1

Tissue Specificity

Ubiquitously expressed, with a highest expression in skeletal muscle. .

Applications

WB,ICC,IF,Flow Cytometry

Purification

Affinity-chromatography

Concentration

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Function

Catalytic subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate; different complex forms are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis. Involved in regulation of degradative endocytic trafficking and required for the abcission step in cytokinesis, probably in the context of PI3KC3-C2 (PubMed:20643123, PubMed:20208530). Involved in the transport of lysosomal enzyme precursors to lysosomes. Required for transport from early to late endosomes (By similarity). .

Components

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Storage Conditions

Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

101549 MW

Observed Molecular Weight

55 kDa

Fragment

Rabbit IgG

Applications Notes

WB 1:500-1:2000<br>ICC/IF 1:50-1:200<br>FC 1:100

Subcellular Location

Midbody . Late endosome . Cytoplasmic vesicle, autophagosome . As component of the PI3K complex I localized to pre-autophagosome structures. As component of the PI3K complex II localized predominantly to endosomes (Probable). Localizes also to discrete punctae along the ciliary axoneme and to the base of the ciliary axoneme (By similarity). .