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Anti-CD162 Monoclonal Antibody, Carrier-free

Boster Bio Anti-CD162 Monoclonal Antibody catalog # M03674-1. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human.

Product Specifications

Synonyms

Alpha-actinin-2;Alpha-actinin skeletal muscle isoform 2;F-actin cross-linking protein;ACTN2;

Gene Name

Alpha-actinin-2

UniProt

Q14242

Host

Rabbit

Reactivity

Human

Immunogen

A synthesized peptide derived from human CD162 A SLe (x) -type proteoglycan, which through high affinity, calcium-dependent interactions with E-, P- and L-selectins, mediates rapid rolling of leukocytes over vascular surfaces during the initial steps in inflammation. Critical for the initial leukocyte capture.

Clonality

Monoclonal

Clone

Clone: AEHF-19

Tissue Specificity

Expressed in both skeletal and cardiac muscle.

Applications

WB,IHC,ICC,IF,IP

Purification

Affinity-chromatography

Concentration

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Function

F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.

Components

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Storage Conditions

Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

103854 MW

Observed Molecular Weight

103 kDa

Fragment

Rabbit IgG

Applications Notes

WB 1:500-1:1000<br>IHC 1:50-1:200<br>ICC/IF 1:50-1:200<br>IP 1:40

Subcellular Location

Cytoplasm, myofibril, sarcomere, Z line . Colocalizes with MYOZ1 and FLNC at the Z-lines of skeletal muscle.

Protein Name

T-cell surface glycoprotein CD3 zeta chain

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