Anti-Ikaros Monoclonal Antibody, Carrier-free

Boster Bio Anti-Ikaros Monoclonal Antibody catalog # M00531-1. Tested in WB, IHC, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Product Specifications

Synonyms

Zinc finger protein GLI1;Glioma-associated oncogene;Oncogene GLI;GLI1;GLI;

Gene Name

Zinc finger protein GLI1

UniProt

Q13422

Host

Rabbit

Reactivity

Human,Mouse,Rat

Immunogen

A synthesized peptide derived from human Ikaros Transcription regulator of hematopoietic cell differentiation (PubMed:17934067) . Binds gamma-satellite DNA (PubMed:17135265, PubMed:19141594) . Plays a role in the development of lymphocytes, B- and T-cells.

Clonality

Monoclonal

Clone

Clone: AEFA-9

Tissue Specificity

Testis, myometrium and fallopian tube. Also expressed in the brain with highest expression in the cerebellum, optic nerve and olfactory tract. .

Applications

WB,IHC,Flow Cytometry

Purification

Affinity-chromatography

Concentration

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Function

Acts as a transcriptional activator. May regulate the transcription of specific genes during normal development. May play a role in craniofacial development and digital development, as well as development of the central nervous system and gastrointestinal tract. Mediates SHH signaling and thus cell proliferation and differentiation. .

Components

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Storage Conditions

Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

117904 MW

Observed Molecular Weight

40 kDa

Fragment

Rabbit IgG

Applications Notes

WB 1:1000-1:5000<br>IHC 1:100-1:500<br>FC 1:60

Subcellular Location

Cytoplasm. Nucleus. Tethered in the cytoplasm by binding to SUFU. Activation and translocation to the nucleus is promoted by interaction with STK36. Phosphorylation by ULK3 may promote nuclear localization. Translocation to the nucleus is promoted by interaction with ZIC1.