Anti-ALIX Rabbit Monoclonal Antibody, Carrier-free

Boster Bio Anti-ALIX Rabbit Monoclonal Antibody catalog # M01751. Tested in WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Product Specifications

Synonyms

Ezrin;Cytovillin;Villin-2;p81;EZR;VIL2;

Gene Name

Ezrin

UniProt

Q8WUM4

Host

Rabbit

Reactivity

Human,Mouse,Rat

Immunogen

A synthesized peptide derived from human ALIX

Clonality

Monoclonal

Clone

Clone: AEBO-16

Tissue Specificity

Expressed in cerebral cortex, basal ganglia, hippocampus, hypophysis, and optic nerve. Weakly expressed in brain stem and diencephalon. Stronger expression was detected in gray matter of frontal lobe compared to white matter (at protein level). Component of the microvilli of intestinal epithelial cells. Preferentially expressed in astrocytes of hippocampus, frontal cortex, thalamus, parahippocampal cortex, amygdala, insula, and corpus callosum. Not detected in neurons in most tissues studied. .

Applications

WB,Flow Cytometry

Purification

Affinity-chromatography

Concentration

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Function

Probably involved in connections of major cytoskeletal structures to the plasma membrane. In epithelial cells, required for the formation of microvilli and membrane ruffles on the apical pole. Along with PLEKHG6, required for normal macropinocytosis. .

Components

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Storage Conditions

Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

69413 MW

Observed Molecular Weight

96 kDa

Fragment

Rabbit IgG

Applications Notes

WB 1:500-1:2000<br>FC 1:100

Subcellular Location

Apical cell membrane ; Peripheral membrane protein ; Cytoplasmic side . Cell projection . Cell projection, microvillus membrane ; Peripheral membrane protein ; Cytoplasmic side . Cell projection, ruffle membrane ; Peripheral membrane protein ; Cytoplasmic side . Cytoplasm, cell cortex . Cytoplasm, cytoskeleton . Localization to the apical membrane of parietal cells depends on the interaction with MPP5. Localizes to cell extensions and peripheral processes of astrocytes (By similarity). Microvillar peripheral membrane protein (cytoplasmic side). .