Anti-SAE2 / UBA2 Monoclonal Antibody, Carrier-free

Boster Bio Anti-SAE2 / UBA2 Monoclonal Antibody catalog # M03816. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.

Product Specifications

Synonyms

Protein disulfide-isomerase A6;5.3.4.1;Endoplasmic reticulum protein 5;ER protein 5;ERp5;Protein disulfide isomerase P5;Thioredoxin domain-containing protein 7;PDIA6;ERP5, P5, TXNDC7;

Gene Name

Protein disulfide-isomerase A6

UniProt

Q9UBT2

Host

Rabbit

Reactivity

Human,Mouse,Rat

Immunogen

A synthesized peptide derived from human SAE2 / UBA2 The heterodimer acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.

Clonality

Monoclonal

Clone

Clone: AEAI-21

Tissue Specificity

Expressed in platelets (at protein level). .

Applications

WB,IHC,ICC,IF,IP

Purification

Affinity-chromatography

Concentration

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Function

May function as a chaperone that inhibits aggregation of misfolded proteins. Plays a role in platelet aggregation and activation by agonists such as convulxin, collagen and thrombin. .

Components

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Storage Conditions

Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

48121 MW

Observed Molecular Weight

43 kDa

Fragment

Rabbit IgG

Applications Notes

WB 1:500-1:2000<br>IHC 1:50-1:200<br>ICC/IF 1:50-1:200<br>IP 1:50

Subcellular Location

Endoplasmic reticulum lumen . Cell membrane . Melanosome . Identified by mass spectrometry in melanosome fractions from stage I to stage IV (PubMed:12643545). .