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Anti-Pyruvate Dehydrogenase E1 beta subunit PDHB Monoclonal Antibody, Carrier-free

Boster Bio Anti-Pyruvate Dehydrogenase E1 beta subunit PDHB Monoclonal Antibody catalog # M03998. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Product Specifications

Synonyms

Dual specificity mitogen-activated protein kinase kinase 5;MAP kinase kinase 5;MAPKK 5;2.7.12.2;MAPK/ERK kinase 5;MEK 5;MAP2K5;MEK5, MKK5, PRKMK5;

Gene Name

Dual specificity mitogen-activated protein kinase kinase 5

UniProt

P11177

Host

Rabbit

Reactivity

Human,Mouse,Rat

Immunogen

A synthesized peptide derived from human Pyruvate Dehydrogenase E1 beta subunit The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO (2) . It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3) .

Clonality

Monoclonal

Clone

Clone: AEAD-16

Tissue Specificity

Expressed in many adult tissues. Abundant in heart and skeletal muscle.

Applications

WB,IHC,ICC,IF,IP,Flow Cytometry

Purification

Affinity-chromatography

Concentration

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Function

Acts as a scaffold for the formation of a ternary MAP3K2/MAP3K3-MAP3K5-MAPK7 signaling complex. Activation of this pathway appears to play a critical role in protecting cells from stress-induced apoptosis, neuronal survival and cardiac development and angiogenesis. .

Components

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Storage Conditions

Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

50112 MW

Observed Molecular Weight

85 kDa

Fragment

Rabbit IgG

Applications Notes

WB 1:1000-1:5000<br>IHC 1:50-1:200<br>ICC/IF 1:50-1:200<br>IP 1:50<br>FC 1:50

Subcellular Location

Cytoplasm, cytoskeleton . Cell projection, lamellipodium . At the interface between the lamellipodial actin meshwork and the membrane. .

Protein Name

T-cell surface glycoprotein CD3 zeta chain

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