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Anti-GCLM Monoclonal Antibody, Carrier-free

Selected Variant

M02948-carrier-free

Size: 100 µL

Boster Bio Anti-GCLM Monoclonal Antibody catalog # M02948. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Product Specifications

Synonyms

ATP synthase subunit beta, mitochondrial;3.6.3.14;ATP5B;ATPMB, ATPSB;

Gene Name

ATP synthase subunit beta, mitochondrial

UniProt

P48507

Host

Rabbit

Reactivity

Human,Mouse,Rat

Immunogen

A synthesized peptide derived from human GCLM Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 1/2.

Clonality

Monoclonal

Clone

Clone: AEOH-7

Tissue Specificity

Expressed throughout the epithelium of the colon. Also expressed in lamina propria. .

Applications

WB,IHC,ICC,IF,IP,Flow Cytometry

Purification

Affinity-chromatography

Concentration

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Function

Mitochondrial membrane ATP synthase (F (1)F (0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F (1) - containing the extramembraneous catalytic core, and F (0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F (1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F (1). Rotation of the central stalk against the surrounding alpha (3)beta (3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits.

Components

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Storage Conditions

Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

56560 MW

Observed Molecular Weight

59 kDa

Fragment

Rabbit IgG

Applications Notes

WB 1:500-1:2000<br>IHC 1:50-1:200<br>ICC/IF 1:50-1:200<br>IP 1:50<br>FC 1:100

Subcellular Location

Mitochondrion. Mitochondrion inner membrane. Peripheral membrane protein.

Protein Name

T-cell surface glycoprotein CD3 zeta chain

Price

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