Anti-HEC1 NDC80 Monoclonal Antibody, Carrier-free

Boster Bio Anti-HEC1 NDC80 Monoclonal Antibody catalog # M01731. Tested in WB, IP applications. This antibody reacts with Human, Mouse, Rat.

Product Specifications

Synonyms

Eukaryotic translation initiation factor 5A-1;eIF-5A-1;eIF-5A1;Eukaryotic initiation factor 5A isoform 1;eIF-5A;Rev-binding factor;eIF-4D;EIF5A;

Gene Name

Eukaryotic translation initiation factor 5A-1

UniProt

O14777

Host

Rabbit

Reactivity

Human,Mouse,Rat

Immunogen

A synthesized peptide derived from human HEC1 Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore.

Clonality

Monoclonal

Clone

Clone: ACBI-14

Tissue Specificity

Expressed in umbilical vein endothelial cells and several cancer cell lines (at protein level). .

Applications

WB,IP

Purification

Affinity-chromatography

Concentration

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Function

mRNA-binding protein involved in translation elongation. Has an important function at the level of mRNA turnover, probably acting downstream of decapping. Involved in actin dynamics and cell cycle progression, mRNA decay and probably in a pathway involved in stress response and maintenance of cell wall integrity. With syntenin SDCBP, functions as a regulator of p53/TP53 and p53/TP53-dependent apoptosis. Regulates also TNF- alpha-mediated apoptosis. Mediates effects of polyamines on neuronal process extension and survival. May play an important role in brain development and function, and in skeletal muscle stem cell differentiation. Also described as a cellular cofactor of human T-cell leukemia virus type I (HTLV-1) Rex protein and of human immunodeficiency virus type 1 (HIV-1) Rev protein, essential for mRNA export of retroviral transcripts. .

Components

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Storage Conditions

Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

16832 MW

Observed Molecular Weight

68 kDa

Fragment

Rabbit IgG

Applications Notes

WB 1:1000-1:5000<br>IP 1:50

Subcellular Location

Cytoplasm. Nucleus. Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side. Nucleus, nuclear pore complex. Hypusine modification promotes the nuclear export and cytoplasmic localization and there was a dynamic shift in the localization from predominantly cytoplasmic to primarily nuclear under apoptotic inducing conditions.