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Anti-ZAP70 Monoclonal Antibody, Carrier-free

Boster Bio Anti-ZAP70 Monoclonal Antibody catalog # M00754-3. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human.

Product Specifications

Synonyms

G2/mitotic-specific cyclin-B1;CCNB1;CCNB;

Gene Name

G2/mitotic-specific cyclin-B1

UniProt

P43403

Host

Rabbit

Reactivity

Human

Immunogen

A synthesized peptide derived from human ZAP70 The Syk family protein tyrosine kinase Zap-70 is expressed in T and NK cells and plays a critical role in mediating T cell activation in response to T cell receptor (TCR) engagement. Following TCR engagement, Zap-70 is rapidly phosphorylated on several tyrosine residues through autophosphorylation and transphosphorylation by the Src family tyrosine kinase Lck.

Clonality

Monoclonal

Clone

Clone: ACAF-26

Tissue Specificity

Ubiquitous. Expressed at high levels in testis.

Applications

WB,IHC,ICC,IF,IP,Flow Cytometry

Purification

Affinity-chromatography

Concentration

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Function

Essential for the control of the cell cycle at the G2/M (mitosis) transition. .

Components

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Storage Conditions

Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

48337 MW

Observed Molecular Weight

100 kDa

Fragment

Rabbit IgG

Applications Notes

WB 1:500-1:1000<br>IHC 1:50-1:200<br>ICC/IF 1:50-1:200<br>IP 1:50<br>FC 1:50

Subcellular Location

Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome.

Protein Name

T-cell surface glycoprotein CD4

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