Anti-Phospho-Histone H3 (T3) H3F3A Monoclonal Antibody, Carrier-free

Boster Bio Anti-Phospho-Histone H3 (T3) H3F3A Monoclonal Antibody catalog # MP06819. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human.

Product Specifications

Synonyms

Red fluorescent protein eqFP611;GFP-like chromoprotein;

Gene Name

Red fluorescent protein eqFP611

UniProt

P84243

Host

Rabbit

Reactivity

Human

Immunogen

A synthesized peptide derived from human Phospho-Histone H3 (T3) H3 Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Clonality

Monoclonal

Clone

Clone: AEO-8

Tissue Specificity

Highly and selectively expressed by undifferentiated rather than differentiated embryonic stem cells (ESC). Levels rapidly diminish as soon as ESC's differentiate (at protein levels). Expressed in almost all epithelial cell membranes but not on mesodermal or neural cell membranes. Found on the surface of adenocarcinoma. .

Applications

WB,IHC,ICC,IF,Flow Cytometry

Purification

Affinity-chromatography

Concentration

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Function

Pigment protein. .

Components

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Storage Conditions

Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

26053 MW

Observed Molecular Weight

15 kDa

Fragment

Rabbit IgG

Applications Notes

WB 1:500-1:2000<br>IHC 1:50-1:200<br>ICC/IF 1:50-1:200<br>FC 1:50

Subcellular Location

Lateral cell membrane ; Single-pass type I membrane protein . Cell junction, tight junction . Colocalizes with CLDN7 at the lateral cell membrane and tight junction. .