Anti-Androgen Receptor (AR-V7 specific) Rabbit Monoclonal Antibody, Carrier-free

Boster Bio Anti-Androgen Receptor (AR-V7 specific) Rabbit Monoclonal Antibody catalog # M00542-3. Tested in WB, IHC applications. This antibody reacts with Human.

Product Specifications

Synonyms

Androgen receptor;Dihydrotestosterone receptor;Nuclear receptor subfamily 3 group C member 4;AR;DHTR, NR3C4;

Gene Name

Androgen receptor

UniProt

C0JKD3

Host

Rabbit

Reactivity

Human

Immunogen

A synthesized peptide derived from human Androgen Receptor (AR-V7 specific)

Clonality

Monoclonal

Clone

Clone: DHH-1

Tissue Specificity

Isoform 2 is mainly expressed in heart and skeletal muscle (PubMed:15634333). Isoform 3 is expressed by basal and stromal cells of prostate (at protein level) (PubMed:19244107). .

Applications

WB,IHC

Purification

Affinity-chromatography

Concentration

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Function

Steroid hormone receptors are ligand-activated transcription factors that regulate eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Transcription factor activity is modulated by bound coactivator and corepressor proteins. Transcription activation is down-regulated by NR0B2. Activated, but not phosphorylated, by HIPK3 and ZIPK/DAPK3. .

Components

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Storage Conditions

Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

99188 MW

Observed Molecular Weight

54 kDa

Fragment

Rabbit IgG

Applications Notes

WB 1:500-1:2000<br>IHC 1:50-1:200

Subcellular Location

Nucleus . Cytoplasm . Predominantly cytoplasmic in unligated form but translocates to the nucleus upon ligand-binding. Can also translocate to the nucleus in unligated form in the presence of RACK1. .