Anti-Stromal interaction molecule 1 STIM1 Rabbit Monoclonal Antibody, Carrier-free

Boster Bio Anti-Stromal interaction molecule 1 STIM1 Rabbit Monoclonal Antibody catalog # M00312. Tested in WB, IHC, IP applications. This antibody reacts with Human, Mouse, Rat.

Product Specifications

Synonyms

Stromal interaction molecule 1;STIM1;GOK;

Gene Name

Stromal interaction molecule 1

UniProt

Q13586

Host

Rabbit

Reactivity

Human,Mouse,Rat

Immunogen

A synthesized peptide derived from human Stromal interaction molecule 1

Clonality

Monoclonal

Clone

Clone: HEC-19

Tissue Specificity

Ubiquitously expressed in various human primary cells and tumor cell lines. .

Applications

WB,IHC,IP

Purification

Affinity-chromatography

Concentration

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Function

Plays a role in mediating store-operated Ca (2+) entry (SOCE), a Ca (2+) influx following depletion of intracellular Ca (2+) stores. Acts as Ca (2+) sensor in the endoplasmic reticulum via its EF-hand domain. Upon Ca (2+) depletion, translocates from the endoplasmic reticulum to the plasma membrane where it activates the Ca (2+) release-activated Ca (2+) (CRAC) channel subunit, TMEM142A/ORAI1. Involved in enamel formation. .

Components

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Storage Conditions

Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

77423 MW

Observed Molecular Weight

85 kDa

Fragment

Rabbit IgG

Applications Notes

WB 1:1000-1:2000<br>IHC 1:50-1:200<br>IP 1:50

Subcellular Location

Cell membrane; Single-pass type I membrane protein. Endoplasmic reticulum membrane; Single-pass type I membrane protein. Cytoplasm, cytoskeleton. Translocates from the endoplasmic reticulum to the cell membrane in response to a depletion of intracellular calcium and is detected at punctae corresponding to junctions between the endoplasmic reticulum and the cell membrane. Associated with the microtubule network at the growing distal tip of microtubules.