Anti-Interferon Receptor alpha IFNAR1 Rabbit Monoclonal Antibody, Carrier-free

Boster Bio Anti-Interferon Receptor alpha IFNAR1 Rabbit Monoclonal Antibody catalog # M00306. Tested in WB application. This antibody reacts with Human.

Product Specifications

Synonyms

Interferon alpha/beta receptor 1;IFN-R-1;IFN-alpha/beta receptor 1;Cytokine receptor class-II member 1;Cytokine receptor family 2 member 1;CRF2-1;Type I interferon receptor 1;IFNAR1;IFNAR;

Gene Name

Interferon alpha/beta receptor 1

UniProt

P17181

Host

Rabbit

Reactivity

Human

Immunogen

A synthesized peptide derived from human Interferon Receptor alpha

Clonality

Monoclonal

Clone

Clone: AF-9

Tissue Specificity

IFN receptors are present in all tissues and even on the surface of most IFN-resistant cells. Isoform 1, isoform 2 and isoform 3 are expressed in the IFN-alpha sensitive myeloma cell line U266B1. Isoform 2 and isoform 3 are expressed in the IFN-alpha resistant myeloma cell line U266R. Isoform 1 is not expressed in IFN-alpha resistant myeloma cell line U266R. .

Applications

Purification

Affinity-chromatography

Concentration

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Function

Component of the receptor for type I interferons, including interferons alpha, IFNB1 and IFNW1 (PubMed:2153461, PubMed:7665574, PubMed:10049744, PubMed:14532120, PubMed:15337770, PubMed:21854986). Functions in general as heterodimer with IFNAR2 (PubMed:7665574, PubMed:10049744, PubMed:21854986). Type I interferon binding activates the JAK-STAT signaling cascade, and triggers tyrosine phosphorylation of a number of proteins including JAKs, TYK2, STAT proteins and the IFNR alpha- and beta- subunits themselves (PubMed:7665574, PubMed:21854986). Can form an active IFNB1 receptor by itself and activate a signaling cascade that does not involve activation of the JAK-STAT pathway (By similarity). .

Components

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Storage Conditions

Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

63525 MW

Observed Molecular Weight

62 kDa

Fragment

Rabbit IgG

Applications Notes

WB 1:500-1:2000

Subcellular Location

Isoform 1: Cell membrane ; Single- pass type I membrane protein . Late endosome . Lysosome . Interferon binding triggers internalization of the receptor from the cell membrane into endosomes and then into lysosomes. .