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Anti-Cytokeratin 5 (C-term) KRT5 Rabbit Monoclonal Antibody, Carrier-free

Selected Variant

M00398-2-carrier-free

Size: 100 µL

Boster Bio Anti-Cytokeratin 5 (C-term) <a href="https://www.bosterbio.com/anti-cytokeratin-5-krt5-antibody-a00398-1-boster.html" style="color:#EA8D28">KRT5 Rabbit Monoclonal Antibody</a> catalog # M00398-2. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse.

Product Specifications

Synonyms

Keratin, type II cytoskeletal 5;58 kDa cytokeratin;Cytokeratin-5;CK-5;Keratin-5;K5;Type-II keratin Kb5;KRT5;

Gene Name

Keratin, type II cytoskeletal 5

UniProt

P13647

Host

Rabbit

Reactivity

Human,Mouse

Immunogen

A synthesized peptide derived from human Cytokeratin 5 (C-term)

Clonality

Monoclonal

Clone

Clone: AGD-11

Tissue Specificity

Released primarily from activated T lymphocytes.

Applications

WB,IHC,ICC,IF

Purification

Affinity-chromatography

Concentration

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Function

Plays a key role in the response to DNA damage. May have a role in checkpoint signaling during mitosis. Enhances TP53- mediated transcriptional activation. .

Components

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Storage Conditions

Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

62378 MW

Observed Molecular Weight

43 kDa

Fragment

Rabbit IgG

Applications Notes

WB 1:500-1:2000<br>IHC 1:50-1:200<br>ICC/IF 1:50-1:200

Subcellular Location

Nucleus. Chromosome, centromere, kinetochore. Associated with kinetochores. Both nuclear and cytoplasmic in some cells. Recruited to sites of DNA damage, such as double stand breaks. H4K20me2 is required for efficient localization to double strand breaks and removal of proteins that have a high affinity for H4K20me2 such as L3MBTL1 and KDM4A is needed.

Protein Name

T-cell surface glycoprotein CD4

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