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Anti-Topoisomerase II alpha TOP2A Rabbit Monoclonal Antibody, Carrier-free

Selected Variant

M00953-1-carrier-free

Size: 100 µL

Boster Bio Anti-Topoisomerase II alpha TOP2A Rabbit Monoclonal Antibody catalog # M00953-1. Tested in WB, IHC, IP applications. This antibody reacts with Human, Mouse, Rat.

Product Specifications

Synonyms

DNA topoisomerase 2-alpha;5.99.1.3;DNA topoisomerase II, alpha isozyme;TOP2A;TOP2;

Gene Name

DNA topoisomerase 2-alpha

UniProt

P11388

Host

Rabbit

Reactivity

Human,Mouse,Rat

Immunogen

A synthesized peptide derived from human Topoisomerase II alpha

Clonality

Monoclonal

Clone

Clone: FB-20

Tissue Specificity

Expressed in the excretory system, thyroid gland and Wilms tumors.

Applications

WB,IHC,IP

Purification

Affinity-chromatography

Concentration

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Function

Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks. Essential during mitosis and meiosis for proper segregation of daughter chromosomes. May play a role in regulating the period length of ARNTL/BMAL1 transcriptional oscillation (By similarity). .

Components

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Storage Conditions

Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

174385 MW

Observed Molecular Weight

28 kDa

Fragment

Rabbit IgG

Applications Notes

WB 1:500-1:2000<br>IHC 1:50-1:200<br>IP 1:50

Subcellular Location

Cytoplasm . Nucleus, nucleoplasm . Generally located in the nucleoplasm.

Protein Name

Glycogen synthase kinase-3 beta (gsk3b)

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