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Anti-Wilms Tumor Protein WT1 Rabbit Monoclonal Antibody, Carrier-free

Selected Variant

M00199-1-carrier-free

Size: 100 µL

Boster Bio Anti-Wilms Tumor Protein WT1 Rabbit Monoclonal Antibody catalog # M00199-1. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse.

Product Specifications

Synonyms

Wilms tumor protein;WT33;WT1;

Gene Name

Wilms tumor protein

UniProt

P19544

Host

Rabbit

Reactivity

Human,Mouse

Immunogen

A synthesized peptide derived from human Wilms Tumor Protein

Clonality

Monoclonal

Clone

Clone: CEH-23

Tissue Specificity

Expressed in the kidney and a subset of hematopoietic cells.

Applications

WB,IHC,ICC,IF,Flow Cytometry

Purification

Affinity-chromatography

Concentration

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Function

Transcription factor that plays an important role in cellular development and cell survival. Regulates the expression of numerous target genes, including EPO. Plays an essential role for development of the urogenital system. Recognizes and binds to the DNA sequence 5'-CGCCCCCGC-3'. It has a tumor suppressor as well as an oncogenic role in tumor formation. Function may be isoform-specific: isoforms lacking the KTS motif may act as transcription factors. Isoforms containing the KTS motif may bind mRNA and play a role in mRNA metabolism or splicing. Isoform 1 has lower affinity for DNA, and can bind RNA. .

Components

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Storage Conditions

Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

49188 MW

Observed Molecular Weight

103 kDa

Fragment

Rabbit IgG

Applications Notes

WB 1:500-1:1000<br>IHC 1:50-1:200<br>ICC/IF 1:50-1:200<br>FC 1:50

Subcellular Location

Nucleus. Nucleus, nucleolus. Cytoplasm . Shuttles between nucleus and cytoplasm. .

Protein Name

P2X purinoceptor 1

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