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Anti-Mast Cell Tryptase Rabbit Monoclonal Antibody, Carrier-free

Selected Variant

M08039-carrier-free

Size: 100 µL

Boster Bio Anti-Mast Cell Tryptase Rabbit Monoclonal Antibody catalog # M08039. Tested in WB, IHC, IP applications. This antibody reacts with Human, Mouse, Rat.

Product Specifications

Synonyms

Tryptase alpha/beta-1;Tryptase-1;3.4.21.59;Tryptase I;Tryptase alpha-1;TPSAB1;TPS1, TPS2, TPSB1;

Gene Name

Tryptase alpha/beta-1

UniProt

Q15661

Host

Rabbit

Reactivity

Human,Mouse,Rat

Immunogen

A synthesized peptide derived from human Mast Cell Tryptase

Clonality

Monoclonal

Clone

Clone: FHH-20

Tissue Specificity

Isoform 1 and isoform 2 are expressed in lung, stomach, spleen, heart and skin; in these tissues, isoform 1 is predominant. Isoform 2 is expressed in aorta, spleen, and breast tumor, with highest levels in the endothelial cells of some blood vessels surrounding the aorta, as well as those surrounding the tumor and low levels, if any, in mast cells (at protein level). .

Applications

WB,IHC,IP

Purification

Affinity-chromatography

Concentration

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Function

Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type. May play a role in innate immunity. Isoform 2 cleaves large substrates, such as fibronectin, more efficiently than isoform 1, but seems less efficient toward small substrates (PubMed:18854315). .

Components

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Storage Conditions

Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

30515 MW

Observed Molecular Weight

100 kDa

Fragment

Rabbit IgG

Applications Notes

WB 1:500-1:2000<br>IHC 1:50-1:200<br>IP 1:50

Subcellular Location

Secreted. Released from the secretory granules upon mast cell activation. .

Protein Name

T-cell surface glycoprotein CD3 zeta chain

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